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Analytical Flow Cytometry

Flow Cytometry is an important tool in all aspects of cell biology research. Many different combinations of antibody-based stains, fluorescent protein expression and viability indicators are measured at the single cell level. The core provides access to cytometry equipment and staff with expertise to assist researchers in developing assays. Basic analytical flow cytometry instrumentation at the core includes the BD FACSCelesta and Beckman Coulter Gallios. 

High Parameter Analytical Flow Cytometry

Flow cytometry instrumentation in the core can detect up to 18+ fluorescent parameters at once on the single cell level. The core staff assists researchers in developing high parameter assays, including panel design, instrument set up, assay troubleshooting and data acquisition. Users with complex assays are encouraged to contact core personnel during the planning stages of their experiments.  High parameter instrumentation at the core includes the BD LRSFortessa, BD LSRFortessa X-20, BD FACSymphony A5SE and Cytek Aurora. 

Cell Sorting

The core uses electrostatic droplet-based high-speed cell sorting to isolate a wide variety of cells based on many different combinations of fluorescence markers. Several nozzle size/pressure configurations are available. Up to 21 color cell sorting is provided with a variety of options for collection vessels, including various test tube sizes and multiwell plates. Cell sorting can entail isolation of single cells as well as tube-based collection of up to six simultaneous populations for RNASeq, culture or many other downstream applications.

High Parameter Data Analysis

Analysis of multiparametric cytometry data can be time-consuming and complex. The core provides expertise and access to software with modeling and computational capabilities to analyze high parameter, single cell data sets. Users with 12+ parameter data and imaging cytometry data are encouraged to consult core staff for data analysis. The core has software available for high-dimensional analysis, including FlowJo and FCS Express. 

Custom mAb Conjugations

The core performs conjugations primarily for monoclonal antibodies that are not commercially available as conjugates. The core can prepare 100 microgram-scale antibody conjugations with cell-free QC. Available fluorochromes include AlexaFluor Dyes (488, 647, 555, 594, etc.) and Pacific Blue. Contact Kathryn Cox at kequinta@mdanderson.org for information.

Rates for MD Anderson Cancer Center Faculty

Rates for External Academic Institution Faculty